SHENTEK® Plasmid DNA Quantitation Kit is used to quantitate plasmid DNA residues in gene therapy products, such as the plasmid DNA of lentiviral vector preparation in CAR-T cell therapy. This kit uses duplex real-time PCR technology to detect trace amounts of residual plasmid DNA by means of consensual plasmid sequences, such as replicon of ColE1/pMB1/pBR322/pUC. The target gene (FAM) performs rapid, specific, and reliable quantitative determination of the 102 copies/μL level of residual Plasmid DNA. IPC - Internal Positive Control (VIC) is included in the Plasmid Primer&Probe MIX to evaluate the performance of each PCR reaction. Customers can send DNA sequence of empty plasmid backbones to our technicians for confirmation in advance.